Effect Of 70% Ethanol And 70% Methanol Solvents On Total Phenol Content Of Beetroot Extracts
DOI:
https://doi.org/10.33751/jhss.v10i1.62Keywords:
beetroot tubers;, solvents, ethanol, methanol, total phenolAbstract
Antioxidants are known to be present in beetroot tubers. Antioxidant activity found in plants comes from the amount of phenolic chemicals present, including phenolic acids and polyphenols. To identify the existence of phenolic compounds in plants, the first step that needs to be done is extraction. Extraction is one of the factors that affect the content of bioactive compounds in the extract, because it can directly affect the process of taking phytochemical compounds from plants. The use of solvents during extraction must be in accordance with their polarity. This study's goal is to determine the impact of using 70% ethanol and 70% methanol solvents on beet tuber extracts' overall phenol content. The method used was using Folin-Ciocalteu reagent and measured by UV-Vis spectrophotometry. The outcomes of the study revealed that the overall amount of phenol content with 70% ethanol solvent had higher results compared to 70% methanol solvent. Since the results of the ANOVA test differ just slightly, P≥0.05 indicates that there is no significant difference.
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